Series 9: Molecular Genetics
ISBN : 81-88279-05-6
Year: 2004
HARDBOUND PAGES: VIII + 465
CROWN SIZE Rs. 650/-

FUNDAMENTALS OF GENE, GENOMICS AND GENETIC ENGINEERING

Contents

  • INTRODUCTORY CONCEPT OF GENE
     • Introduction  • How genes were discovered and analyzed  • Mendel discovers the units of heredity • Mendel's first law: The alternative forms of a gene segregate  • Insights from mendel's first law  • Mendel's second law: Different genes are inherited independently  • Mendel's results ignored  • Locating and studying genes on chromosomes  • Different sexes - different chromosomes  • Sex, white eyes, and yellow bodies  • What causes new combinations of genes on the same chromosome?  • Gene interactions : Extensions of Mendel's principles  • Interactions between alleles  • Interactions between genes  • One gene, many effects  • Environmental effects on gene expression  • What genes do  • Wrong genes, wrong enzymes  • Hypothesis revised: One gene - one polypeptide  • What are genes?  • Bacterial invaders: Conclusive proof from DNA in viruses •  DNA : The twisted ladder of inheritance  • DNA : A linear molecule  • Packaging DNA  • Mysteries of heredity unveiled in DNA structure  • How DNA replicates?  • How DNA stores information?  • How DNA generates diversity?  • Altering the thread of life : The power of recombinant DNA research  • Promises and problems in recombinant DNA research  • Reshaping life : The promise of genetic engineering  • Recombinant DNA : Novel problems of safety and ethics  • From DNA to RNA to protein  • RNA : Function and structure  • Transcription : DNA into RNA  • Translation : RNA into protein  • Protein synthesis : Translating genetic messages into the stuff of life  • Gene mutation : A change in base sequence  • How a gene mutation changes physical appearance?  • The origin of mutations  • Regulation of gene activity  • In bacteria  • In people  • Conclusions  • References
  • GENOMICS
     • Introduction  • Functional genomics  • DNA chips  • Molecular bar coding and reverse genetics  • Potential impact on agribusiness  • Microarrays  • Human genomics  • Bacterial genomes • Saccharomyces cerevisiae (yeast) genome  • RNA and protein assays of genome function  • Expressed sequences  • Array hybridization and gene chips  • Use of green fluorescent protein as a reporter of protein synthesis  • Evolution of genomes  • Basic techniques in genomics for clone genes  • Discovery of restriction endonucleases  • Artificial chromosomes  • Construction and screening of DNA libraries  • Genetic selection  • Molecular analysis of DNA , RNA and protein  • Amplification of DNA by PCR  • Physical maps of DNA molecules based on restriction enzyme cleavage sites  • Nucleotide sequences: The ultimate fine structure maps  • Molecular analysis of genes and gene products  • Molecular diagnosis of human diseases  • Human gene therapy  • Transgenic plants and animals  •  Conclusion •  References
  • GENETIC ENGINEERING
     • Introduction  • Techniques of genetic engineering  • Methods of gene isolation, identification and synthesis  • Mapping genes on specific chromosomes  • Genomic library  • Cleaving DNA with restriction enzymes  • Molecular analysis of DNA , RNA and protein  • Detection of RFLP  • DNA sequences  • Mechanical shearing of DNA  • Transcript mapping techniques  • Synthesis of cDNA from mRNA  •  Searching for gene using computers  •  Gene synthesis machines  • Methods of specific gene cloning, transfer and expression  • Amplification of DNA by polymerase chain reaction  • Specific gene transfer  • Expression of induced genes  • Applications of genetic engineering  • Conclusions  • References