Series 8: Biotechnology
ISBN : 81-88279-36-6
Year: 2006
PAPERBACK PAGES: VIII + 289
DEMI SIZE RS. 295/-

MODERN CONCEPTS IN BIOTECHNOLOGY

Contents

  • PROTEOMICS
     • Introduction  • Experimental aspects of proteomics  • Technical inputs in proteomics research  • Current applications of proteomics in varied biological systems  • Plant proteomics research  • NA
    microarrays and proteomics  • Protein folding  • Comparison of protein folding in vitro and with folding in vivo  • Protein folding in biotechnology  • Nature of inclusion bodies  • Solvent system  • Reoxidation of disulphide bonds  • Characterisation of final product  • Future prospects  • Conclusions  • Summary •  Glossary of common terminologies  • References
  • GENOMICS
     • Introduction  • Functional genomics  • DNA chips  • Molecular bar coding and reverse genetics  •  Potential impact on agribusiness  •  Microarrays  •  Human genomics  •  Bacterial genomes  • Saccharomyces cerevisiae (yeast) genome  • RNA and protein assays of genome function  • Expressed sequences  • Array hybridization and gene chips  • Use of green fluorescent protein as a reporter of protein synthesis  • Evolution of genomes  • Basic techniques in genomics for clone genes  • Discovery of restriction endonucleases  • Artificial chromosomes  • Construction and screening of DNA libraries  • Genetic selection  • Molecular analysis of DNA , RNA and protein  • Amplification of DNA by PCR  • Physical maps of DNA molecules based on restriction enzyme cleavage sites  • Nucleotide sequences: The ultimate fine structure maps  • Molecular analysis of genes and gene products  • Molecular diagnosis of human diseases  • Human gene therapy  • Transgenic plants and animals  • Conclusion  • References
  • TRANSPLASTOMICS
     • Introduction  • Chloroplasts in plastid engineering  • Chloroplast structure and evolution  • Huge potential in the tiny plastids  •  Nuclear genome vs plastid genome  •  Plastid genome organizatio
     • Engineering plastid genome of higher plants  • Vectors for plastid transformation  • Manipulation of protein levels  • Methods of chloroplast transformation  • Types of marker genes  • Strategies for alfalfa plastid transformation  •  Means to attain homoplasmic plants  •  Current progress in this field  • Biodegradable plastics  • Molecular pharming  • Unanswered technical questions  • uture prospects  • Conclusion  • References