Series 8: Biotechnology
ISBN : 81-88279-34-X
Year: 2006
PAPERBACK PAGES: VIII + 298
DEMI SIZE Rs. 295/-

ADVANCES IN BIOTECHNOLOGY

CONTENTS

DNA TECHNOLOGY AND PCR APPLICATIONS

  • DNA and its biosynthesis
  • DNA and its applications
  • Recombinant DNA
  • PCR and its applications
  • References

GENETICENGINEERING

  • Introduction
  • Techniques of genetic engineering
  • Methods of gene isolation, identification and synthesis
  • Mapping genes on specific chromosomes
  • Genomic library
  • Cleaving DNA with restriction enzymes
  • Molecular analysis of DNA, RNA and protein
  • Detection of RFLP
  • DNA sequences
  • Mechanical shearing of DNA
  • Transcript mapping techniques
  • Synthesis of cDNA from mRNA
  • Searching for gene using computers
  • Gene synthesis machines
  • Methods of specific gene cloning, transfer and expression
  • Amplification of DNA by polymerase chain reaction
  • Specific gene transfer
  • Expression of induced genes
  • Applications of genetic engineering
  • Conclusions
  • Acknowledgements
  • References