Series 8: Biotechnology
ISBN : 81-88279-33-1
Year: 2006
PAPERBACK PAGES: VIII + 292
DEMI SIZE Rs. 295/-

BASIC CONCEPTS OF BIOTECHNOLOGY

Contents

  • INTRODUCTORY CONCEPT OF GENE
  • DNA STRUCTURE AND ITS BIOSYNTHESIS:  • Introduction  • Types and structure of nucleic acid  • DNA is a double helix  • DNA can occur in different structural forms  • Certain DNA sequences adopt unusual structures  • DNA replication  •  Double-helical DNA and RNA can be denatured  • Genome organisation in prokaryote and eukaryote  • Eukaryotic genomes have several sequence components  • Alu sequences  • Mitochondrial DNA (d-loops) codes for few proteins  • References
  • RNA STRUCTURE AND ITS BIOSYNTHESIS :  • Introduction  • Types of RNA  • RNA polymerases in eukaryotic cells  • RNA processing  • Ribozymes - RNA enzymes  • References
  • PROTEIN STRUCTURE AND ITS BIOSYNTHESIS :  •  Introduction  •  Protein structure  • Protein modelling  • Protein synthesis and the genetic code  • The genetic code  • Protein synthesis Post translational modifications  • References
  • GENETIC REGULATION AND EXPRESSION :  • Introduction  • Many prokaryotic genes are regulated in units called operons  • Repressors and activators - regulatory protein  • The lac and arabinose operon  • Genetic regulation in eukaryotes  • Regulatory strategies in eukaryotes  • Gene alteration  • Gene loss  • Gene amplification  • Gene rearrangement  • Transcriptional control by hormones  • References
  • GENETIC RECOMBINATION IN BACTERIA :  • Introduction  • Transformation  • Transduction  • Conjugation  • Transposition  • Genetic system in bacterium  • F plasmids  • Mechanism of genetic recombination  • Transposons  • References
  • MUTAGENESIS:  • Introduction  • Types of mutagens  • Site specific mutagenesis  • Reversion  •  Testing mutagen  •  DNA repair  •  Genetic analysis of mutants  •  References
  • PLASMIDS AND TRANSPOSONS:  •  Introduction  •  Types of plasmids  •  The purification of plasmid DNA  •  Transfer of plasmid DNA  •  An overview of transposition  •  Types of bacterial transposons  •  References
  • DNA TECHNOLOGY:  • Introduction  • Purification of DNA  • The enzymes to be used for DNA manipulation  • Enzymes to modify ends of DNA molecules  • Vectors for cloning Hybridization techniques  • Gene libraries  • cDNA Library  • References
  • PCR AND ITS APPLICATIONS:  • Introduction  • Designing the oligonucleotide primers for a PCR  • Studying PCR products  • Application of the PCR  • Microarrays  • Molecular markersDrug designing  • Drug targeting l References
  • RECOMBINANT DNA TECHNOLOGY:  • Introduction  • Gene cloning in agriculture  • Recombinant DNA technology in medicine  • Applications in medicine  • Transgenic animals  • References